Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in VitroZejin Cui *1, Pengkun Yuan *2, Zhishan Zhao *1, Fan Zhao 3, Linrong Lu 1,3
1Shanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 2Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, 3Institute of Immunology and Department of Rheumatology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
We present a protocol for retroviral transduction of guide RNA into primary T cells from Cas9 transgenic mice, providing an efficient alternative for gene editing in studying Th17 differentiation.
