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Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors

Polina Golubinskaya¹, Evgeny Ruchko¹, Arina Pikina¹, Anna Barinova¹, Artem Eremeev¹

¹Laboratory of Translational Biomedicine, Cell Biology department,, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

This study describes a method for producing chondrocytic spheroids by aggregating cells into spheroids under low-adhesion conditions using gravity, followed by culturing the resulting spheroids in mini-bioreactors.