ATAC-Seq Optimization for Cancer Epigenetics ResearchMikhala Cooper *1, Atrayee Ray *1, Atrayee Bhattacharya 2, Archana Dhasarathy 1, Motoki Takaku 1
1University of North Dakota School of Medicine and Health Sciences, 2Dana-Farber Cancer Institute
ATAC-seq is a DNA sequencing method that uses the hyperactive mutant transposase, Tn5, to map changes in chromatin accessibility mediated by transcription factors. ATAC-seq enables the discovery of the molecular mechanisms underlying phenotypic alterations in cancer cells. This protocol outlines optimization procedures for ATAC-seq in epithelial cell types, including cancer cells.
