In Vitro Modeling of Down Syndrome Neurogenesis Using Human-Induced Pluripotent Stem CellsVishi Sharma 1, Harish Chhawari 1,2, Pournima Joshi 1,2, Sunita Nehra 1, Nishant Singhal 1,2
1Stem Cell and Neurobiology Lab, National Centre for Cell Science, S.P. Pune University Complex, 2Regional Centre for Biotechnology
This protocol outlines a methodology for recapitulating Down syndrome (DS) impaired neurogenesis using DS human iPSCs. The protocol identified biphasic cell cycle defect as the cause of impaired neurogenesis in Down syndrome. It provides a robust platform for understanding cellular and molecular mechanisms underlying the abnormal neurogenesis associated with DS.
