3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac CellsAndrea Sánchez-Bueno 1, Olalla Iglesias-García 1, Pilar Montero-Calle 1, Juan José Gavira 2, Felipe Prosper 3,4,5, Manuel M. Mazo 1,3
1Biomedical Engineering Program, Enabling Technologies Division, CIMA Universidad de Navarra, and Instituto de Investigación Sanitaria de Navarra (IdiSNA), 2Department of Cardiology, Clínica Universidad de Navarra and Instituto de Investigación Sanitaria de Navarra (IdiSNA), 3Hematology and Cell Therapy Area, Clínica Universidad de Navarra and Instituto de Investigación Sanitaria de Navarra (IdiSNA), 4Centro de Investigación Biomédica en Red de Cáncer (CIBERONC) CB16/12/00489, 5Hemato-Oncology Program, Cancer Division, CIMA Universidad de Navarra
A reproducible method is presented to generate 3D myocardial tissues combining melt electrospinning writing (MEW) polycaprolactone (PCL) scaffolds and fibrin hydrogels with hiPSC-derived cardiomyocytes and fibroblasts. This technique offers precise control over scaffold architecture and can be applied in preclinical drug testing and cardiac disease modeling.
