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TIFR

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

Sudip Mondal¹, Shikha Ahlawat¹, Sandhya P. Koushika^1,2

¹Neurobiology, NCBS-TIFR, ²Department of Biological Sciences, TIFR

A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport. We demonstrate the use of this device and show examples of different types of data collected from different model systems.

Bioengineering

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Jyoti Dubey^1,2,3,4, Sudip Mondal^1,5, Sandhya P. Koushika²

¹National Centre for Biological Sciences, TIFR, ²Department of Biological Sciences, TIFR, ³InSTEM, ⁴Manipal Academy of Higher Education, ⁵Department of Mechanical Engineering, The University of Texas at Austin

The protocol describes a simple microfluidic chip design and microfabrication methodology used to grow C. elegans in presence of a continuous food supply for up to 36 h. The growth and imaging device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular processes during development for several days.