Highly Resolved Intravital Striped-illumination Microscopy of Germinal CentersZoltan Cseresnyes *1,2, Laura Oehme *3, Volker Andresen 4, Anje Sporbert 2, Anja E. Hauser *3,5, Raluca Niesner *1
1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
