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Universitätsmedizin Berlin

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons

¹Institute of Integrative Neuroanatomy, NeuroCure Cluster of Excellence, Charité Universitätmedizin

Cortical networks are controlled by a small, but diverse set of inhibitory interneurons. Functional investigation of interneurons therefore requires targeted recording and rigorous identification. Described here is a combined approach involving whole-cell recordings from single or synaptically-coupled pairs of neurons with intracellular labeling, post-hoc morphological and immunocytochemical analysis.

Neuroscience

Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Paul Turko¹, Keenan Groberman¹, Toralf Kaiser², Yuchio Yanagawa³, Imre Vida¹

¹Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, ²Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, ³Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.