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AUT University and KODE Biotech Ltd

2 ARTICLES PUBLISHED IN JoVE

Biology

Optimized PCR-based Detection of Mycoplasma

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals.

Biology

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

Deborah A. Blake¹, Nicolai V. Bovin², Dan Bess¹, Stephen M. Henry¹

¹Biotechnology Research Institute, AUT University and KODE Biotech Ltd, ²Shemyakin Institute of Bioorganic Chemistry RAS, Moscow, Russia

Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.