Heterogeneity Mapping of Protein Expression in Tumors using Quantitative ImmunofluorescenceDana Faratian 1, Jason Christiansen 2, Mark Gustavson 2, Christine Jones 2, Christopher Scott 2, InHwa Um 1, David J. Harrison 1
1Division of Pathology, University of Edinburgh, 2HistoRx Inc.
Here we describe a method to quantify molecular heterogeneity in histological sections of tumor material using quantitative immunofluorescence, image analysis, and a statistical measure of heterogeneity. The method is intended for use in clinical biomarker development and analysis.
