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Inova Health System

2 ARTICLES PUBLISHED IN JoVE

Biology

Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks

Rohini Mehta^1,2, Ancha Baranova^1,2,3, Aybike Birerdinc^1,2

¹Molecular and Microbiology Department and Center for the Study of Genomics in Liver Diseases, George Mason University, ²Translational Research Institute, Inova Health System, ³Research Center for Medical Genetics RAMS

Here we present a low cost, durable cryotolerant device for sample retrieval from Dewar tanks filled with liquid nitrogen. The ease of construction and modular design of the device makes the process of sample retrieval from cryogenic tanks safe and easy.

Cancer Research

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Dave Mitchell^*1,2, Allison L. Hunt^*1,4, Kelly A. Conrads^1,2, Brian L. Hood^1,2, Sasha C. Makohon-Moore^1,2, Christine Rojas¹, G. Larry Maxwell^1,3,4, Nicholas W. Bateman^1,2,3, Thomas P. Conrads^1,3,4

¹Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center, ²The Henry M. Jackson Foundation for the Advancement of Military Medicine, ³The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University, ⁴Women’s Health Integrated Research Center, Inova Women’s Service Line, Inova Health System

This protocol describes a high-throughput workflow for artificial intelligence-driven segmentation of pathology-confirmed regions of interest from stained, thin tissue section images for enrichment of histology-resolved cell populations using laser microdissection. This strategy includes a novel algorithm enabling the transfer of demarcations denoting cell populations of interest directly to laser microscopes.