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Katholieke Universiteit Leuven

3 ARTICLES PUBLISHED IN JoVE

Medicine

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction

Ryan Hodges^1,2, Masayuki Endo¹, Andre La Gerche³, Elisenda Eixarch^4,5, Philip DeKoninck¹, Vessilina Ferferieva³, Jan D'hooge³, Euan M. Wallace², Jan Deprest¹

¹Division Woman and Child, Department Women, University Hospitals Leuven, ²The Ritchie Centre, Monash Institute of Medical Research, Department of Obstetrics and Gynaecology, Monash University, Victoria, Australia, ³Department of Cardiovascular Sciences, Katholieke Universiteit Leuven, ⁴Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), ⁵Maternal-Fetal Medicine Department, ICGON, Hospital Clínic, Universitat de Barcelona, Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER)

We describe examination of fetal cardiac function with contemporary functional fetal echocardiography and fetoplacental Doppler ultrasound using the VisualSonics VEVO 2100 microultrasound in a surgically induced model of intrauterine fetal growth restriction in a rabbit.

Medicine

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Laura Ordovás^*1, Ruben Boon^*1, Mariaelena Pistoni¹, Yemiao Chen¹, Rangarajan Sambathkumar¹, Nicky Helsen¹, Jolien Vanhove¹, Pieter Berckmans¹, Qing Cai¹, Kim Vanuytsel¹, Susanna Raitano¹, Catherine M. Verfaillie¹

¹Stem Cell Institute, Katholieke Universiteit Leuven

Here we report a rapid and efficient gene editing method based on RMCE in the AAVS1 locus of human Pluripotent Stem Cells (hPSCs) that improves upon previously described systems. Using this technique, isogenic lines can be rapidly and reliably generated for proper comparative studies, facilitating transgenesis-mediated research with hPSCs.

Neuroscience

Investigating Object Representations in the Macaque Dorsal Visual Stream Using Single-unit Recordings

Irene Caprara¹, Peter Janssen^1,2, Maria C. Romero¹

¹Laboratorium voor Neuro-en Psychofysiologie, Katholieke Universiteit Leuven, ²The Leuven Brain Institute

A detailed protocol to analyze object selectivity of parieto-frontal neurons involved in visuomotor transformations is presented.