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Aichi Medical University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Kouji Komatsu¹, Akira Iwase^2,3, Tomohiko Murase², Satoru Masubuchi¹

¹Department of Physiology, Aichi Medical University, ²Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, ³Department of Maternal and Perinatal Medicine, Nagoya University Hospital

Ovarian tissue cultures can be used as models of follicle development, ovulation, and follicle atresia and indicate regulatory mechanisms of dynamic ovarian processes.

Immunology and Infection

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Md. Monirul Islam¹, Umme Salma², Takayuki Irahara², Eizo Watanabe², Naoshi Takeyama²

¹Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), ²Department of Emergency and Critical Care Medicine, Aichi Medical University

We present a protocol for a modified sandwich enzyme-linked immunosorbent assay technique to quantitatively measure two components of neutrophil extracellular trap remnants, myeloperoxidase conjugated-DNA and neutrophil elastase conjugated-DNA complexes,derived from activated neutrophils.