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Niigata University Graduate School of Medical and Dental Sciences

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Perrine Inquimbert¹, Martin Moll², Tatsuro Kohno³, Joachim Scholz²

¹Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique (CNRS), ²Departments of Anesthesiology and Pharmacology, Columbia University , ³Department of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences

Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.

Medicine

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

Eriko Katsuta¹, Masanori Oshi¹, Omar M. Rashid^2,3,4,5, Kazuaki Takabe^1,6,7,8,9,10

¹Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, ²Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, ³Department of Surgery, Massachusetts General Hospital, ⁴Department of Surgery, University of Miami Miller School of Medicine, ⁵Department of Surgery, Nova Southeastern University School of Medicine, ⁶Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, ⁷Department of Breast Surgery and Oncology, Tokyo Medical University, ⁸Department of Surgery, Yokohama City University, ⁹Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, ¹⁰Department of Surgery, Fukushima Medical University

We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.

Biology

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Yuko Miyagawa¹, Kazunori Nagasaka¹, Kaoru Yamawaki^2,3, Yutaro Mori^2,3, Tatsuya Ishiguro², Kei Hashimoto¹, Ryoko Koike¹, Siho Fukui¹, Takeru Sugihara¹, Takayuki Ichinose¹, Haruko Hiraike¹, Koichiro Kido¹, Koji Okamoto³, Takayuki Enomoto², Takuya Ayabe¹

¹Department of Obstetrics and Gynecology, Teikyo University School of Medicine, ²Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, ³Devision of Cancer Differentiation, National Cancer Center Research Institute

Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro.