Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)Samira Samtleben 1, Juliane Jaepel 1,2, Caroline Fecher 1, Thomas Andreska 1, Markus Rehberg 3, Robert Blum 1
1Institute for Clinical Neurobiology, University of Wuerzburg, 2Department of Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University of Munich
Targeted-esterase induced dye loading (TED) supports the analysis of intracellular calcium store dynamics by fluorescence imaging. The method bases on targeting of a recombinant Carboxylesterase to the endoplasmic reticulum (ER), where it improves the local unmasking of synthetic low-affinity Ca2+ indicator dyes in the ER lumen.
