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bengbu medical college

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy

Liangfei Niu¹, Cong Wang¹, Kaile Zhang^1,2, Miaomiao Kang^1,2, Rui Liang¹, Xiaonan Zhang¹, Bo Yan¹

¹Shanghai Public Health Clinical Center, Fudan University, ²School of Life Sciences, Bengbu Medical College

This protocol describes a technique for visualizing macrophage behavior and death in embryonic zebrafish during Mycobacterium marinum infection. Steps for the preparation of bacteria, infection of the embryos, and intravital microscopy are included. This technique may be applied to the observation of cellular behavior and death in similar scenarios involving infection or sterile inflammation.