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The Ragon Institute of MGH, MIT and Harvard

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

Filippos Porichis^*1, Meghan G. Hart^*1, Jennifer Zupkosky¹, Lucie Barblu^1,2, Daniel E. Kaufmann^1,2

¹The Ragon Institute of MGH, MIT and Harvard, ²Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM)

We developed an in vitro assay to investigate the role of immunoregulatory pathways in the regulation of cytokine secretion by HIV-specific CD4 T cells.