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National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ)

1 ARTICLES PUBLISHED IN JoVE

Biology

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Richard Gabriel¹, Ina Kutschera¹, Cynthia C Bartholomae¹, Christof von Kalle¹, Manfred Schmidt¹

¹Department of Translational Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ)

Linear-amplification mediated (LAM)-PCR is a method developed to identify the exact positions of integrating viral vectors in the genome. The technique has evolved to be the superior method to study clonal dynamics in gene therapy patients, biosafety of novel vector technologies, T-cell diversity, cancer stem cell models, etc.