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University Hospital of South Manchester

2 ARTICLES PUBLISHED IN JoVE

Medicine

An Isolated Working Heart System for Large Animal Models

Matthew A. Schechter¹, Kevin W. Southerland¹, Bryan J. Feger¹, Dean Linder Jr.¹, Ayyaz A. Ali², Linda Njoroge¹, Carmelo A. Milano¹, Dawn E. Bowles¹

¹Department of Surgery, Duke University Medical Center, ²Department of Cardiothoracic Surgery, University Hospital of South Manchester

Most studies involving the Langendorff apparatus use small animal models due to the increased complexity of systems for larger mammals. We describe a Langendorff system for large animal models that allows for use across a range of species, including humans, and relatively easy data acquisition.

Neuroscience

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

Alba C. de Luca¹, Alessandro Faroni², Adam J. Reid^2,3

¹Centre for Neuroprosthesis, EPFL | STI | IMT/IBI | LSBI, ²Blond McIndoe Research Laboratories, Institute of Inflammation & Repair, The University of Manchester, ³University Hospital of South Manchester

Dorsal root ganglia (DRG) are structures containing the sensory neurons of the peripheral nervous system. When dissociated, they can be co-cultured with SC-like adipose-derived stem cells (ASC), providing a valuable model to study in vitro nerve regeneration and myelination, mimicking the in vivo environment at the injury site.