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University of Science and Technology of China

10 ARTICLES PUBLISHED IN JoVE

Biology

Using the optokinetic response to study visual function of zebrafish

Su-Qi Zou¹, Wu Yin¹, Ming-Jing Zhang¹, Chun-Rui Hu¹, Yu-Bin Huang¹, Bing Hu¹

¹Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China (USTC)

Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults^1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.

Medicine

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

Ronald X. Xu¹, Kun Huang², Ruogu Qin¹, Jiwei Huang¹, Jeff S. Xu¹, Liya Ding², Urmila S. Gnyawali³, Gayle M. Gordillo³, Surya C. Gnyawali^3,4, Chandan K. Sen³

¹Department of Biomedical Engineering, The Ohio State University, ²Department of Biomedical Informatics, The Ohio State University, ³Comprehensive Wound Center, The Ohio State University, ⁴Department of Surgery, The Ohio State University

A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.

Neuroscience

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

Su-Qi Zou¹, Chen Tian¹, Su-Tie Du¹, Bing Hu¹

¹Laboratory of Neurodevelopement and Repair, University of Science and Technology of China

We introduce an efficient method to retrograde label retinal ganglion cells (RGCs) in adult zebrafish.

Engineering

Evaluating Plasmonic Transport in Current-carrying Silver Nanowires

Mingxia Song¹, Arnaud Stolz¹, Douguo Zhang², Juan Arocas¹, Laurent Markey¹, Gérard Colas des Francs¹, Erik Dujardin³, Alexandre Bouhelier¹

¹Laboratoire Interdisciplinaire Carnot de Bourgogne CNRS-UMR 6303, Université de Bourgogne, ²Department of Optics and Optical Engineering, University of Science and Technology of China, ³CEMES, CNRS-UPR 8011

Silver nanowires can simultaneously transport electrons and optical information in the form of surface plasmons. A procedure is described here to realize such a shared circuitry and the limitations at propagating both information carriers are evaluated.

Immunology and Infection

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Ke Yang^*1,2,3, Jiandong Wu^*3,4, Ling Zhu¹, Yong Liu¹, Michael Zhang⁵, Francis Lin^3,4,6,7

¹Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, ²University of Science and Technology of China, ³Department of Physics and Astronomy, University of Manitoba, ⁴Department of Biosystems Engineering, University of Manitoba, ⁵Seven Oaks General Hospital, ⁶Department of Immunology, University of Manitoba, ⁷Department of Biological Sciences, University of Manitoba

This article provides the detailed method of performing a rapid neutrophil chemotaxis assay by integrating the on-chip neutrophil isolation from whole blood and the chemotaxis test on a single microfluidic chip.

Behavior

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Lifen Jiang^*1,2, Yinpeng Zhan^*2,3, Yan Zhu^2,3

¹School of Life Science, University of Science and Technology of China, ²State key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, ³University of Chinese Academy of Sciences

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

Developmental Biology

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Mengrou Liu^*1, Chunsen Zhu^*1, Shun Bai^*2, Xin Li¹, Kaiqiang Fu¹, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ²Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China

Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

Immunology and Infection

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Meng Wang^*1, Yuanyuan Huang^*1, Wangpeng Gu^2,3, Haikun Wang¹

¹CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, ²School of Life Science, University of Science and Technology of China, ³State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences

This paper describes protocols of evaluating Tfh and GC B response in mouse model of influenza virus infection.

Engineering

Multimodal 3D Printing of Phantoms to Simulate Biological Tissue

Canzhen Ma^*1,2, Shuwei Shen^*1,2, Guangli Liu¹, Siyue Guo^1,2, Buyun Guo^1,2, Jialuo Li^1,2, Kuiming Huang^1,2, Yidan Zheng¹, Pengfei Shao^1,2, Erbao Dong^1,2, Jiaru Chu¹, Ronald X. Xu^1,2,3

¹Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, ²Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes, University of Science and Technology of China, ³Department of Biomedical Engineering, The Ohio State University

Spin coating, polyjet printing, and fused deposition modeling are integrated to produce multilayered heterogeneous phantoms that simulate structural and functional properties of biological tissue.

Medicine

Avoiding Ischemia Reperfusion Injury in Liver Transplantation

Yixi Zhang^*1,2,3, Changjun Huang^*1,2,3, Weiqiang Ju^*1,2,3, Qiang Zhao^1,2,3, Maogen Chen^1,2,3, Linhe Wang^1,2,3, Dongping Wang^1,2,3, Linwei Wu^1,2,3, Zebin Zhu⁴, Shanzhou Huang⁵, Zhiheng Zhang^1,2,3, Caihui Zhu^1,2,3, Rongxing Xie^1,2,3, Yinghua Chen^1,2,3, Anbin Hu^1,2,3, Xiaofeng Zhu^1,2,3, Zhiyong Guo^1,2,3, Xiaoshun He^1,2,3

¹Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, ²Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, ³Guangdong Provincial International Cooperation Base of Science and Technology, ⁴Organ Transplant Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, ⁵Department of General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences

Presented here is a protocol to provide a step-by-step ischemia-free liver transplantation protocol under ex situ normothermic machine perfusion (37 °C) of human livers from donors to recipients.