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University of Oklahoma

6 ARTICLES PUBLISHED IN JoVE

Neuroscience

Electrophysiology of Scorpion Peg Sensilla

Elizabeth D. Knowlton¹, Douglas D. Gaffin¹

¹Department of Zoology, University of Oklahoma

This article describes an electrophysiological method for isolating chemical stimulation to individual sensilla via extracellular, tip-recordings under mineral oil.

Biology

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Wei Rao¹, Ning Pan¹, Zhibo Yang¹

¹Department of Chemistry and Biochemistry, University of Oklahoma

Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis.

Bioengineering

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

Christopher A. Neal¹, Jennifer G. Nelson-Brantley¹, Michael S. Detamore², Hinrich Staecker¹, Adam J. Mellott³

¹Department of Otolaryngology, University of Kansas Medical Center, ²Stephenson School of Biomedical Engineering, University of Oklahoma, ³Department of Plastic Surgery, University of Kansas Medical Center

The goal of this protocol is to demonstrate an effective method to decellularize and decalcify mouse cochleae for utilization as scaffolds for tissue engineering applications.

Engineering

Magnet Assisted Composite Manufacturing: A Flexible New Technique for Achieving High Consolidation Pressure in Vacuum Bag/Lay-Up Processes

Maya Pishvar^*1, Mehrad Amirkhosravi^*1, M. Cengiz Altan¹

¹School of Aerospace and Mechanical Engineering, University of Oklahoma

A new technique for applying consolidation pressure on the vacuum bag lay-up to fabricate composite laminates is described. The goal of this protocol is to develop a simple, cost-effective technique to improve the quality of laminates fabricated by the wet lay-up vacuum bag method.

Chemistry

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Shawna J. Standke¹, Devon H. Colby¹, Ryan C. Bensen¹, Anthony W. G. Burgett¹, Zhibo Yang¹

¹Department of Chemistry and Biochemistry, University of Oklahoma

An integrated cell manipulation platform is developed for use in conjunction with a single-probe mass spectrometry setup for the on-line analysis of individual suspension cells under ambient conditions.

Environment

In Situ Soil Moisture Sensors in Undisturbed Soils

Todd G. Caldwell¹, Michael H. Cosh², Steven R. Evett³, Nathan Edwards⁴, Heather Hofman⁵, Bradley G. Illston⁶, Tilden Meyers⁷, Marina Skumanich⁸, Kent Sutcliffe⁹

¹Nevada Water Science Center, U.S. Geological Survey, ²Agricultural Research Service, Hydrology and Remote Sensing Lab., U.S. Department of Agriculture, ³Agricultural Research Service, Conservation and Production Research Lab., U.S. Department of Agriculture, ⁴South Dakota State University, ⁵National Water and Climate Center, U.S. Department of Agriculture, ⁶University of Oklahoma, ⁷Air Resources Lab, National Oceanic and Atmospheric Administration, ⁸National Integrated Drought Information System, National Oceanic and Atmospheric Administration, ⁹Snow Survey Staff, U.S. Department of Agriculture

The determination of soil water content is a critical mission requirement for many state and federal agencies. This protocol synthesizes multi-agency efforts to measure soil water content using buried in situ sensors.