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Flemish Institute for Technological Research (VITO)

2 ARTICLES PUBLISHED IN JoVE

Medicine

Fundus Photography as a Convenient Tool to Study Microvascular Responses to Cardiovascular Disease Risk Factors in Epidemiological Studies

Patrick De Boever^1,2, Tijs Louwies^1,2, Eline Provost^1,2, Luc Int Panis^1,3, Tim S. Nawrot^2,4

¹Environmental Risk and Health, Flemish Institute for Technological Research (VITO), ²Centre for Environmental Sciences, Hasselt University, ³Transportation Research Institute, Hasselt University, ⁴Department of Public Health, Occupational and Environmental Medicine, Leuven University

Retinal image analysis is an unobtrusive procedure for visualizing the microcirculation. The impact of cardiovascular disease risk factors can result in changes of retinal vessel calibers. The procedures to acquire fundus images and the steps for calculating the vessel calibers are described.

Medicine

Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Marco Giallombardo^*1,2, Jorge Chacártegui Borrás^*2,3, Marta Castiglia⁴, Nele Van Der Steen³, Inge Mertens^5,6, Patrick Pauwels^7,3, Marc Peeters^8,3, Christian Rolfo^2,3

¹Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, ²Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA), ³Center for Oncological Research (CORE), Antwerp University, ⁴Department of Surgical, Oncological and Oral Sciences, Section of Medical Oncology, University of Palermo, ⁵Flemish Institute for Technological Research (VITO), ⁶CORE, Campus Groenenborger, Antwerp University, ⁷Molecular Pathology, Pathology Department, Antwerp University Hospital (UZA), ⁸Oncology Department, Antwerp University Hospital (UZA)

This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.