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U.S Environmental Protection Agency

3 ARTICLES PUBLISHED IN JoVE

Environment

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples

G. Shay Fout¹, Jennifer L. Cashdollar¹, Eunice A. Varughese¹, Sandhya U. Parshionikar², Ann C. Grimm¹

¹National Exposure Research Laboratory, U.S Environmental Protection Agency, ²Technical Support Center, Office of Ground Water and Drinking Water, U.S Environmental Protection Agency

EPA Method 1615 uses an electropositive filter to concentrate enteroviruses and noroviruses in environmental and drinking waters. This manuscript describes the procedure for collecting samples for Method 1615 analyses.

Environment

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

G. Shay Fout¹, Jennifer L. Cashdollar¹

¹National Exposure Research Laboratory, U.S. Environmental Protection Agency

Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

Environment

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

G. Shay Fout¹, Jennifer L. Cashdollar¹, Shannon M. Griffin¹, Nichole E. Brinkman¹, Eunice A. Varughese¹, Sandhya U. Parshionikar²

¹National Exposure Research Laboratory, U.S. Environmental Protection Agency, ²Technical Services Center, Office of Ground Water and Drinking Water, U.S. Environmental Protection Agency

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.