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University of Shanghai for Science and Technology

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Lindsay N. Nail¹, Dawei Zhang^2,3, Jessica L. Reinhard¹, Melissa A. Grunlan^1,2

¹Department of Biomedical Engineering, Texas A&M University, ²Department of Material Science and Engineering, Texas A&M University, ³Institute of Advanced Materials and Technology, University of Science & Technology Beijing

Scaffolds capable of fitting within cranio-maxillofacial (CMF) bone defects while exhibiting osteoconductivity and bioactivity are of interest. This protocol describes the preparation of a shape memory scaffold based on polycaprolactone diacrylate (PCL-DA) using a solvent-casting particulate-leaching (SCPL) method employing a fused salt template and application of a bioactive polydopamine coating.

Biochemistry

Real-time Tracking of DNA Fragment Separation by Smartphone

Chunxian Tao¹, Bo Yang¹, Zhenqing Li¹, Dawei Zhang¹, Yoshinori Yamaguchi^1,2,3

¹Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, ²Department of Applied Physics, Graduate School of Engineering, Osaka University, ³East China University of Science and Technology, Department of Physics Faculty of Science

Traditional slab gel electrophoresis (SGE) experiments require a complicated apparatus and high chemical consumption. This work presents a protocol that describes a low-cost method to separate DNA fragments within a short timeframe.

Bioengineering

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Wenwen Dong¹, Chunxian Tao¹, Bo Yang¹, Erika Miyake², Zhenqing Li¹, Dawei Zhang¹, Yoshinori Yamaguchi^1,2

¹Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, ²Graduate School of Engineering, Osaka University

This protocol describes how to build a continuous-flow-polymerase chain system based on a microfluidic chip and how to build a capillary electrophoresis system in the lab. It presents a simple method for the analysis of nucleic acids in the lab.