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Inner Mongolia Agricultural University

1 ARTICLES PUBLISHED IN JoVE

Environment

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Yan-Ru Zhang^1,2, Hai-Li Qiao³, Li-Li Ren¹, Rong Wang⁴, Peng-Fei Lu¹

¹The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, ²School of Forestry, Inner Mongolia Agricultural University, ³Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, ⁴School of Forestry, Fujian Agriculture and Forestry University

In order to observe ultrastructure of insect sensilla, scanning and transmission electron microscopy (SEM and TEM, respectively) sample preparation protocol were presented in the study. Tween 20 was added into the fixative to avoid sample deformation in SEM. Fluorescence microscopy was helpful for improving slicing accuracy in TEM.