Tokushima Bunri University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye

Yoko Tominaga¹, Makiko Taketoshi¹, Naoko Maeda¹, Takashi Tominaga¹

¹Laboratory for Neural Circuit Systems, Institute of Neuroscience, Tokushima Bunri University

We introduce a reproducible and stable optical recording method for brain slices using voltage-sensitive dye. The article describes voltage-sensitive dye staining and recording of optical signals using conventional hippocampal slice preparations.