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Shanxi Medical University

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure

Honghui Mao^*1,2, Xiuchao Wang^*1,3, Wen Chen⁴, FengYu Liu⁵, You Wan⁵, Sanjue Hu¹, Junling Xing^1,6

¹Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, ²Department of Toxicology, School of Public Health, ShanXi Medical University, ³Department of Psychology, Fourth Military Medical University, ⁴Department of Neurobiology, School of Basic Medical Sciences, Advanced Innovation Center for Human Brain Protection, Capital Medical University, ⁵Neuroscience Research Institute, Key Lab for Neuroscience, Ministry of Education/National Health Commission, Peking University, ⁶Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University

Single-fiber recording is an effective electrophysiological technique that is applicable to the central and peripheral nervous systems. Along with the preparation of intact DRG with the attached sciatic nerve, the mechanism of conduction failure is examined. Both protocols improve the understanding of the peripheral nervous system's relationship with pain.

Neuroscience

Handcrafted Silicone Coated Filament for Mice Middle Cerebral Artery Occlusion Models

Min Zhang^1,2, Yitong Yuan³, Ruochen Du³, Chunli Wen¹, Shiqin Lin², Yaqiong Zhang², Zhenyu Xiang⁴, Fengyun Hu¹, Chunfang Wang³

¹Department of Neurology, Fifth Hospital of Shanxi Medical University, Shanxi Provincial People's Hospital, ²Department of Neurology, Taiyuan Central Hospital, Shan Xi Medical University, ³Experimental Animal Center, Shanxi Medical University, ⁴Collaborative Innovation Center for Molecular Imaging of Precision Medicine, Shan Xi Medical University

This protocol describes a straightforward method for creating coated filaments for the middle cerebral artery occlusion (MCAO) model in mice using silicone, nylon sutures, and syringe needles. This method allows for the production of filaments with a consistent diameter and various silicone wrapping lengths tailored to experimental needs.