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University of Basel Children's Hospital

4 ARTICLES PUBLISHED IN JoVE

Biochemistry

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Xinzhou Zhu¹, Andrea Zelmer¹, Sven Wellmann^1,2

¹University Children's Hospital Basel (UKBB), University of Basel, ²Department of Clinical Research, University of Basel

Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.

Medicine

Electrophysiological Measurement of Noxious-evoked Brain Activity in Neonates Using a Flat-tip Probe Coupled to Electroencephalography

Noemi Klarer^*1, Hanna Rickenbacher^*1, Severin Kasser¹, Antoinette Depoorter², Sven Wellmann¹

¹Division of Neonatology, University of Basel Children's Hospital, ²Divison of Neuropediatrics and Developmental Medicine, University of Basel Children's Hospital

Measuring pain in non-verbal patients is a challenge. In this study we combine EEG recording with stimulation using a flat-tip probe to detect noxious-evoked brain activity in an objective manner.

Behavior

An Objective and Child-friendly Assessment of Arm Function by Using a 3-D Sensor

Xing Chen¹, Detlef Wolf¹, Juliane Siebourg-Polster², Christian Czech³, Ulrike Bonati^4,5, Dirk Fischer^4,5, Omar Khwaja⁶, Martin Strahm¹

¹Data Science, Roche Pharmaceutical Research and Early Development Informatics, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., ²Translational Technologies and Bioinformatics, Pharmaceutical Sciences, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., ³Biomarker Experimental Medicine, Neuroscience, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., ⁴Division of Neuropediatrics, University of Basel Children's Hospital, ⁵Department of Neurology, University of Basel Hospital, ⁶Translational Medicine, Neuroscience and Rare Diseases, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd.

An objective measure of muscle functions is challenging especially in children. Based on a commercially available digital 3-D sensor, a child-friendly gaming test was developed to assess upper limb function for clinical trials.

Medicine

Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy

Sara Nagy^1,2, Simone Schmidt^1,2, Patricia Hafner^1,3, Andrea Klein^1,4,5, Daniela Rubino-Nacht¹, Vanya Gocheva¹, Oliver Bieri⁶, Carole Vuillerot⁷, Ulrike Bonati^1,2, Dirk Fischer^1,2,3

¹Division of Pediatric Neurology, University Children's Hospital Basel (UKBB), University of Basel, ²Department of Neurology, University Hospital Basel, University of Basel, ³Division of Neurology, University Clinic of Medicine, Cantonal Hospital Baselland, Bruderholz, ⁴Division of Pediatric Neurology, CHUV, ⁵Division of Neuropaediatrics, Inselspital, University Children's Hospital Bern, ⁶Department of Radiology, Division of Radiological Physics, University Hospital Basel, University of Basel, ⁷Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, L'Escale, Service de Médecine Physique et de Réadaptation Pédiatrique

The aim of this study is to present the most reliable clinical outcome measures and their correlations with quantitative muscle MRI in ambulant patients with Duchenne muscular dystrophy.