Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming CellsDae Hwan Kim *1, Long-Hui Cui *2, Ha-Rim Seo 2, Hyung Joon Joo 2, Seung-Cheol Choi 2, Do-Sun Lim 2, Kyu Back Lee 1
1School of Biomedical Engineering, Korea University, 2Department of Cardiology, Korea University Anam Hospital
Here, we present a protocol for the fabrication of gradient nanopattern plates via thermal nanoimprinting and the method of screening responses of human endothelial progenitor cells to the nanostructures. By using the described technology, it is possible to produce a scaffold that can manipulate cell behavior by physical stimuli.
