Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic GenesAlicia Masters 1,2, Minjeong Kang 1,3,4, Morgan McCaw 1,3, Jacob D. Zobrist 1,3,5, William Gordon-Kamm 2, Todd Jones 2, Kan Wang 1,3
1Department of Agronomy, Iowa State University, 2Department of Applied Science and Technology, Corteva Agriscience, 3Crop Bioengineering Center, Iowa State University, 4Interdepartmental Plant Biology Major, Iowa State University, 5Interdepartmental Genetics and Genomics Major, Iowa State University
Plant morphogenic genes can be used to improve genetic transformation of recalcitrant genotypes. Described here is an Agrobacterium-mediated genetic transformation (QuickCorn) protocol for three important public maize inbred lines.
