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Korea Research Institute of Standards and Science (KRISS)

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Jina Lee^*1,2, Joo-Eun Lee^*3, Seil Kim^1,4,5, Dukjin Kang¹, Hee Min Yoo¹

¹Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), ²College of Pharmacy, Chungnam National University (CNU), ³Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), ⁴Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), ⁵Department of Bio-Analysis Science, University of Science & Technology (UST)

Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.

JoVE Core

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Ana C. Quevedo¹, Emily Guggenheim¹, Sophie M. Briffa¹, Jessica Adams^2,3, Stephen Lofts², Minjeong Kwak⁴, Tae Geol Lee⁴, Colin Johnston⁵, Stephan Wagner⁶, Timothy R. Holbrook⁶, Yves U. Hachenberger⁷, Jutta Tentschert⁷, Nicholas Davidson¹, Eugenia Valsami-Jones¹

¹School of Geography, Earth and Environmental Sciences, University of Birmingham, ²UK Centre for Ecology and Hydrology, ³Natural England, ⁴Center for Nanosafety Metrology, Korea Research Institute of Standards and Science (KRISS), ⁵Department of Materials, University of Oxford, ⁶Department of Analytical Chemistry, Helmholtz-Centre for Environmental Research, ⁷Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR)

This study presents the benchmarking results for an interlaboratory comparison (ILC) designed to test the standard operating procedure (SOP) developed for gold (Au) colloid dispersions characterized by ultraviolet-visible Spectroscopy (UV-Vis), amongst six partners from the H2020 ACEnano project for sample preparation, measurement, and analysis of the results.