Experimental and Data Analysis Workflow for Soft Matter NanoindentationGiuseppe Ciccone 1, Mariana Azevedo Gonzalez Oliva 1, Nelda Antonovaite 2, Ines Lüchtefeld 3, Manuel Salmeron-Sanchez 1, Massimo Vassalli 1
1Centre for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, 2Optics 11 life, 3Laboratory of Biosensors and Bioelectronics, ETH Zürich
The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.
