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The Johns Hopkins School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

Lisa Grant^*1, Eunyoung Yi^*1, Juan D. Goutman², Elisabeth Glowatzki¹

¹Department of Otolaryngology-Head and Neck Surgery., The Johns Hopkins School of Medicine, ²Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas

Whole-cell patch-clamp recordings from auditory nerve fiber dendrites at the inner hair cell ribbon synapse in the mammalian cochlea.

Cancer Research

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Shun Ishiyama^1,2,3,4, Casey Kissel⁵, Xin Guo¹, Alexis Howard⁶, Harumi Saeki⁷, Tomoaki Ito⁸, Polina Sysa-Shah⁹, Hajime Orita¹⁰, Kazuhiro Sakamoto⁴, Kathleen Gabrielson^1,2

¹Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, ²Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, ³Department of Surgery, Johns Hopkins University School of Medicine, ⁴Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, ⁵Program for Comparative Medicine, Gene Therapy Program, Perelman School of Medicine, University of Pennsylvania, ⁶Tuskegee College of Veterinary Medicine, ⁷Department of Human Pathology, Juntendo University Faculty of Medicine, ⁸Department of Surgery, Juntendo University Shizuoka Hospital, Juntendo University School of Medicine, ⁹Department of Urology, The Johns Hopkins School of Medicine, ¹⁰Department of Gastroenterology and Minimally Invasive Surgery, Juntendo University Faculty of Medicine

Here, a syngeneic orthotopic implantation followed by an amputation procedure of the osteosarcoma with spontaneous pulmonary metastasis that can be used for preclinical investigation of metastasis biology and development of novel therapeutics is described.