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Ciudad de la Habana, Cuba

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

Diana Pauly¹, Pablo A. Chacana², Esteban G. Calzado³, Björn Brembs⁴, Rüdiger Schade⁵

¹Center for Biological Security, Robert Koch-Institute, ²CICVyA - INTA Castelar, Instituto de Virología, ³Center of Molecular Immunology, Ciudad de la Habana, Cuba, ⁴Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology, Free University of Berlin, ⁵Institut of Pharmacology, Charité-University Medicine of Berlin

This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.