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Oregon State University

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

Shih-Heng Su¹, Katie A. Clark², Nicole M. Gibbs¹, Susan M. Bush¹, Patrick J. Krysan¹

¹Horticulture Department, University of Wisconsin-Madison, ²Department of Zoology, Oregon State University

The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.