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The University of Iowa

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

Andrew J. Shepherd¹, Durga P. Mohapatra^1,2

¹Department of Pharmacology, The University of Iowa, ²Department of Anesthesia, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa

Immunocytochemical identification of peripheral sensory nerve fiber subtypes (and detection of protein expression therein) are key to the understanding of molecular mechanisms underlying peripheral sensation. Here we describe methods for preparation of peripheral/visceral tissue samples, such as skin and limb bones, for specific immunostaining of peripheral sensory nerve fibers.

Intratracheal Aerosolization of Viral Vectors to Newborn Pig Airways

Ashley L. Cooney¹, Patrick L. Sinn¹

¹Stead Family Department of Pediatrics, the Pappajohn Biomedical Institute, and the Center for Gene Therapy, The University of Iowa

To maximize the potential benefits of pulmonary gene therapy, widespread and uniform topical delivery of a viral vector across the surface epithelium is an important goal. Here, we demonstrate an aerosolization technique using a microsprayer positioned intratracheally to deliver a viral vector to newborn pig airways.