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Uppsala University

2 ARTICLES PUBLISHED IN JoVE

Biology

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Caroline Kampf¹, IngMarie Olsson¹, Urban Ryberg¹, Evelina Sjöstedt¹, Fredrik Pontén¹

¹Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University

Tissue microarrays allows for an efficient method to gain concurrent information from a multitude of tissues. Representative parts of tissues are assembled into a single paraffin block. Sections from the block are used for immunohistochemistry and analysis of protein expression patterns. Digital scanning generates corresponding images for distribution of data.

Medicine

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Konstantin Galichanin^1,2, Nooshin Talebizadeh², Per Söderberg²

¹St. Erik's Eye Hospital, Karolinska Institutet, ²Gullstrand lab, Section for Ophthalmology, Department of Neuroscience, Uppsala University

Cataract is the leading cause of blindness in the world. Solar ultraviolet radiation (UVR) is the main risk factor for cataract development. An animal model of far UVR-B induced cataract was developed. In this article we describe methods for investigation of cataract formation: exposure to UVR, quantitative RT-PCR and immunohistochemistry.