Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical MicroscopeKevin G. Phillips 1,2, Sandra M. Baker-Groberg 1, Owen J.T. McCarty 1,3
1Department of Biomedical Engineering, Oregon Health & Science University, School of Medicine, 2Department of Dermatology, Oregon Health & Science University, School of Medicine, 3Department of Cell & Developmental Biology, Division of Hematology & Medical Oncology, Knight Cancer Institute, Oregon Health & Science University, School of Medicine
We describe the use of a standard optical microscope to perform quantitative measurements of cellular mass, volume, and density through a combination of bright field and differential interference contrast imagery.
