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3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Bioluminescent Bacterial Imaging In Vivo

Chwanrow K. Baban^*1, Michelle Cronin^*1, Ali R. Akin¹, Anne O'Brien¹, Xuefeng Gao¹, Sabin Tabirca¹, Kevin P. Francis¹, Mark Tangney¹

¹Cork Cancer Research Centre, BioSciences Institute, University College Cork

This article describes the administration of lux-tagged bacteria to mice and subsequent in vivo analysis using IVIS bioluminescence imaging.

Medicine

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Nicholas M. Bernthal¹, Brad N. Taylor², Jeffrey A. Meganck², Yu Wang³, Jonathan H. Shahbazian³, Jared A. Niska¹, Kevin P. Francis², Lloyd S. Miller^3,4

¹Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles (UCLA), ²PerkinElmer, ³Department of Dermatology, Johns Hopkins University School of Medicine, ⁴Department of Medicine, Division of Infectious Diseases, Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine

Combined optical and μCT imaging in a mouse model of orthopaedic implant infection, utilizing a bioluminescent engineered strain of Staphylococcus aureus, provided the capability to noninvasively and longitudinally monitor the dynamics of the bacterial infection, as well as the corresponding inflammatory response and anatomical changes in the bone.

Cancer Research

Utilizing ¹⁸F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Milica Momcilovic¹, Sean T. Bailey², Jason T. Lee³, Charles Zamilpa³, Anthony Jones³, Gihad Abdelhady¹, James Mansfield⁴, Kevin P. Francis⁵, David B. Shackelford¹

¹Division of Pulmonary and Critical Care Medicine, University of California Los Angeles David Geffen School of Medicine, ²University of North Carolina at Chapel Hill, ³Department of Molecular and Medical Pharmacology, University of California Los Angeles, ⁴Andor Technology, ⁵Division of Orthopaedic Surgery, University of California Los Angeles David Geffen School of Medicine

In this protocol, we describe how to utilize [¹⁸F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography (¹⁸F-FDG PET/CT) imaging to measure the tumor metabolic response to the targeted therapy MLN0128 in a Kras/Lkb1 mutant mouse model of lung cancer and coupled imaging with high resolution ex vivo autoradiography and quantitative histology.