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University of Verona, Verona, Italy

3 ARTICLES PUBLISHED IN JoVE

JoVE Journal

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

Elisa Gecchele^*1, Matilde Merlin^*1, Annalisa Brozzetti², Alberto Falorni², Mario Pezzotti¹, Linda Avesani¹

¹Department of Biotechnology, University of Verona, Verona, Italy, ²Department of Internal Medicine, University of Perugia, Perugia, Italy

In this study the expression of a target human recombinant protein in different production platforms was compared. We focused on traditional fermenter-based cultures and on plants, describing the set-up of each system and highlighting, on the basis of the reported results, the inherent limits and advantages for each platform.

Genetics

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Silvia Dal Santo^*1, Mauro Commisso^*1, Erica D'Incà¹, Andrea Anesi^1,2, Matteo Stocchero³, Sara Zenoni¹, Stefania Ceoldo¹, Giovanni B. Tornielli¹, Mario Pezzotti¹, Flavia Guzzo¹

¹Biotechnology Department, University of Verona, ²Lab. of Bioorganic Chemistry, Physics Department, University of Trento, ³S-IN Soluzioni Informatiche

This article describes the application of untargeted metabolomics, transcriptomics and multivariate statistical analysis to grape berry transcripts and metabolites in order to gain insight into the terroir concept, i.e., the impact of the environment on berry quality traits.

Bioengineering

Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes

Mattia Santoni^*1, Edoardo Bertini^*1, Roberta Zampieri¹, Anna Cuccurullo¹, Mauro Commisso¹, Elisa Gecchele¹, Linda Avesani¹

¹Department of Biotechnology, University of Verona

Here, we present a protocol to produce an oral vaccine candidate against Type 1 diabetes in an edible plant.