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Mount Sinai Hospital, Toronto

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Janet M. Denbeigh^1,2, Brian A. Nixon^1,2, Mira C. Puri^1,2,3, F. Stuart Foster^1,2

¹Department of Medical Biophysics, University of Toronto, ²Sunnybrook Research Institute, ³Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto

Here, we present a protocol to inject ultrasound microbubble contrast agents into living, isolated late-gestation stage murine embryos. This method enables the study of perfusion parameters and of vascular molecular markers within the embryo using contrast-enhanced high-frequency ultrasound imaging.