Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular SignalingChristian Berens 1,2, Stephanie Bisle 3, Leonie Klingenbeck 3, Anja Lührmann 3
1Department Biologie, Friedrich-Alexander-Universität, 2Institut für Molekulare Pathogenese, Friedrich-Loeffler-Institut, 3Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen
The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.
