Isolation of Exosomes from the Plasma of HIV-1 Positive IndividualsKateena Addae Konadu 1, Ming Bo Huang 1, William Roth 1, Wendy Armstrong 2, Michael Powell 1, Francois Villinger 3,4, Vincent Bond 1
1Department of Microbiology, Biochemistry, Immunology, Morehouse School of Medicine, 2Department of Medicine, Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 4Yerkes National Primate Research Center
Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.
