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Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Jörg Flemmig¹, Pauline Schwarz¹, Ingo Bäcker², Anna Leichsenring², Franziska Lange², Jürgen Arnhold¹

¹Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, ²Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig

This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.