Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode ArraysStefan Hahnewald 1,2, Marta Roccio 1,2, Anne Tscherter 3, Jürg Streit 3, Ranjeeta Ambett 1,2, Pascal Senn 1,2,4
1Department of Otorhinolaryngology, Inselspital and University of Bern, 2Department of Clinical Research, Inselspital and University of Bern, 3Department of Physiology, University of Bern, 4Department of Clinical Neurosciences, Service ORL & HNS, University Hospital Geneva
We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.
