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Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Angelika Schmidt¹, Szabolcs Éliás^*1, Rubin N. Joshi^*1, Jesper Tegnér¹

¹Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory

This protocol describes the reproducible generation and phenotyping of human induced regulatory T cells (iTregs) from naïve CD4⁺ T cells in vitro. Different protocols for FOXP3 induction allow for the study of specific iTreg phenotypes obtained with respective protocols.