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University of Oxford, Inorganic Chemistry Laboratory

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Protein Film Infrared Electrochemistry Demonstrated for Study of H₂ Oxidation by a [NiFe] Hydrogenase

Philip A. Ash^*1, Ricardo Hidalgo^*1, Kylie A. Vincent¹

¹Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory

Here, we describe a technique, protein film infrared electrochemistry, which allows immobilized redox proteins to be studied spectroscopically under direct electrochemical control at a carbon electrode. Infrared spectra of a single protein sample can be recorded at a range of applied potentials and under a variety of solution conditions.