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Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC)

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Rosa Gómez-Villafuertes^*1,2,3, Lucía Paniagua-Herranz^*1,2,3, Sergio Gascon^*4,5, David de Agustín-Durán^1,2,3, María de la O Ferreras^1,2,3, Juan Carlos Gil-Redondo^1,2,3, María José Queipo^1,2,3, Aida Menendez-Mendez^1,2,3, Ráquel Pérez-Sen^1,2,3, Esmerilda G. Delicado^1,2,3, Javier Gualix^1,2,3, Marcos R. Costa⁶, Timm Schroeder⁷, María Teresa Miras-Portugal^1,2,3, Felipe Ortega^1,2,3

¹Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine, Complutense University, ²University Institute for Neurochemistry Research (IUIN), ³Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), ⁴Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilians University Munich, ⁵Toxicology and Pharmacology Department, Faculty of Veterinary medicine, Complutense University, ⁶Brain Institute, Federal University of Rio Grande do Norte, ⁷Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

Neuroscience

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Isabel Mateos-White¹, Jaime Fabra-Beser¹, David de Agustín-Durán¹, Cristina Gil-Sanz¹

¹Estructura de Recerca Interdisciplinar en Biotecnología y Biomedicina (ERI BIOTECMED), Departamento de Biología Celular, Biología Funcional y Antropología Física, Universidad de Valencia

Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest.