Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass ExperimentsMorgan R. McPherson 1, Peng Wang 1, Ellen L. Marsh 1, Robert B. Mitchell 2, Daniel P. Schachtman 1
1Department of Agronomy and Horticulture and Center for Plant Science Innovation, University of Nebraska-Lincoln, 2USDA-ARS, Wheat, Sorghum and Forage Research Unit, University of Nebraska-Lincoln East Campus
Excavation of plant roots from the field as well as processing of samples into endosphere, rhizosphere, and soil are described in detail, including DNA extraction and data analysis methods. This paper is designed to enable other laboratories to use these techniques for the study of soil, endosphere, and rhizosphere microbiomes.
