Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in RatFrancis A. M. Manno *1, Sinai H. C. Manno *2,3,4, Victor Ma 5, Fernando A. Barrios 1, William C Cho 5, Shuk Han Cheng 2,3,6, Condon Lau 4
1Instituto de Neurobiología, Universidad Nacional Autónoma de México, México, 2State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, Hong Kong, 3Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, 4Department of Physics, City University of Hong Kong, Hong Kong, 5Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, 6Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong
Here, we present a protocol to establish a replicable conductive hearing loss induction via surgical tympanic membrane puncture and verification by otoscope visualization and behavioral assessment by clap startle.
